Yes, I have had that done several times. They numb your neck with Novocaine or something similar and then go in with the fine needle and take several samples. It isn't too bad at all. You have to stay very still while they put the needle in, and you might feel some pressure. I have a very large substernal goiter and will be seeing a surgeon in a couple of weeks. Had the darned goiter forever, now it has moved my trachea off to the side, so I think it's time for it to go.